MOLECULAR ANALYSIS (NGS & PCR/DGGE) OF RUMINAL BACTERIA OF GOATS DURING SHIFT FROM FORAGE TO CONCENTRATE DIET.

D.J.GRILLI; N. SOHAEFER; C. PEREYRA; S. RUIZ; L. PEREYRA; L.QUIROGA; S. VERNOLA; G.N. ARENAS; J.MRAZEJ

Mendoza

Congreso; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

High-grain feeding used in the animal production is known to affect the host ruminal bacteria, but our understanding of consequent changes in goats is limited.Considering this, the goal of this study was to evaluate the ruminal bacteria dynamics during adaptation to a high concentrate diet in 4 fistulated goats, which were fed for 30 days with alfalfahay (AH). Later on, 40% of corn (C) was joined, maintaining this diet (AH/C) for 20 days. Samples of rumen contents were taken on days 2, 10 and 20 for both diets, with their respective pH measurement. The sequence of16S rDNA for the analysis of bacterial populations was amplified by two culture independent methods(PCR/DGGE and NGS). In the beginning, the measurement of the pH was taken, which showed a significant decrease, but without exceeding the limit established for acute and subacute ruminal acidosis. PCR/DGGE analysis showed that bacterial population were grouped considering the diet but not strictly reflect the sampling day, indicating some stability in the ruminal bacteria core, despite high individual variation. The NGS analysis showed a significant decrease (p