THE KIDNEY IN HYPERTENSIVE-ZINC DEFICIENT RATS

ARRANZ C; TOMAT AL; GIRGULSKY C; VEIRAS L; INSERRA F; BALASZCZUK AM; COSTA MA

Milán

Congreso; XV Eurpean Meeting on Hypertension; 2005

European Society of Hypertension

Poster Session 7: Renal Aspects Saturday, June 18, 2005 Abstract: P1.246 Citation: Journal of Hypertension Volume 23, Supplement 2, June 2005, page S90 THE KIDNEY IN HYPERTENSIVE-ZINC DEFICIENT RATS C. Arranz1, A. Tomat1, L. Girgulsky1, L. Veiras1, F. Inserra2, A.M. Balaszczuk1, M.A. Costa1 1 FFyB. University of Buenos Aires, IQUIMEFA-CONICET, Argentina 2 ININCA-CONICET, Buenos Aires, Argentina Objective: In previous studies we demonstrated that moderate ZD during growth significantly increased arterial blood pressure and decreased activity of vascular and renal nitric oxide system. The purpose of this study was to evaluate the histological and functional renal parameters in rats with a moderate zinc deficiency during growth. Methods: Three-week-old weaned male Wistar rats were divided into two dietary groups and fed either a moderate zinc-deficient diet (ZD, 8 ppm zinc) or a control diet (C, 30 ppm zinc) for 60 days. Rats were born from mothers that fed a rat chow containing a concentration of zinc recommended for the period of pregnancy and lactation. 60 days after the initiation of dietary treatment systolic blood pressure (SBP), creatinine clearence (CLcreat) were measured. Then the animals were sacrificed to determine expression of renal alpha-smooth muscle actin (a-SMA) and renal histological structure. Results: See the table.                                                                                                                                                                                                                                                                                                                                                                                             Group                              C                                    ZD                                                                                                                                                                                                                                                                                                                                                                                                    SBP                              129±2                          149±2*     Clcreat                      0.52±0.05                 0.41±0.03*  Yuxtamedullar Glomerulus number     6.1±0.4                       4.4±0.2*    Glomerular capillary area/total area           67.2±1.7                    54.9±1.8*   a-SMA                                0.207±0.011                                0.216±0.033                                                                                                                                                                                                                                                                                                                                                                                             *p < 0.001 vs DC.             Conclusion: Moderate zinc deficiency in growing animals induced an increase in SBP and a decrease on glomerular filtration rate associated to a diminished number of glomerulus and filtration area. However, we did not find changes in the renal a-SMA expression.