INVESTIGADORES
TOMAT Analia Lorena
congresos y reuniones científicas
Título:
Prenatal and postnatal zinc deficiency programs endotelial dysfunction in male rats. (Trabajo premiado)
Autor/es:
MENDES-GARRIDO F; JURIOL L; GOBETTO MN; WENK G; BRUNELLO F; CANIFFI C; ELESGARAY R; TOMAT AL; ARRANZ C
Lugar:
Foz de Iguazú
Reunión:
Congreso; 1st PanAmerican Congress of Physiological Sciences; 2014
Institución organizadora:
Physiology Societies
Resumen:
Moderate zinc deficiency during
intrauterine and postnatal growth induces an increase in systolic blood
pressure (SBP), cardiac and renal alterations. We evaluated aortic nitric oxide (NO) production and vascular function
in adult male rats exposed to fetal and postnatal zinc deficiency. Female
Wistar rats received during pregnancy up to weaning low(L:8ppm) or control(C:30ppm)
zinc diet. After weaning, male offspring fed low (l) or control (c) zinc diet
during 60 days (Cc,Ll,Lc). At day 81, we measured NO synthase (NOS) activity (pmol
14C-L-citrulline/g.tissue.min, 14C-L-Arginine method) in thoracic
aorta. In aortic rings suspended in Krebs solution and preconstricted with
phenylephrine (10-5M), we evaluated the relaxation with
acetylcholine(ACh,10-10-10-3M) or sodium nitroprusside(SNP,NO
donor,10-13-10-5M). To evaluate endothelium-independent
relaxation, ACh protocol was also performed in endothelium-denuded rings.
Statistics: One way ANOVA, Bonferroni post-test,*p<0.01 vs Cc (n=6/group). Ll
and Lc rats showed a decrease in aortic NOS activity (Cc:225±5, Ll:172±4*,
Lc:160±8*). In aortic rings, ACh maximal relaxation was 21% and 15% lower in Ll
and Lc respectively, compared to Cc, and it was completely abolished in
endothelium-denuded rings. No differences were observed in SNP relaxation. Zinc
deficiency during fetal and postnatal life programs an impaired vasodilator
capacity due to a lower endothelial NO production that could contribute to SBP
elevation in adult life. Supported by:UBA-CONICET