CHARACTERIZATION OF IGG ISOTYPES AGAINST SEVERAL Trypanosoma cruzi ANTIGENS AND THEIR PROFILES ALONG CHAGAS HEART DISEASE STAGES

LUZ MARIA PEVERENGO; LUZ MARIA RODELES; MIGUEL VICCO; ESTEFANIA PROCHETTO; GABRIEL CABRERA; IVÁN BONTEMPI; GIULIANA LUPI; CAROLINA ROLDÁN; LIHUE GONZALEZ; IVÁN MARCIPAR

Buenos aires

Congreso; Reunión conjunta de sociedades de biociencias; 2017

SAIC, SAIB, SAI, SAA, SAB, SAB, SAFE, SAFIS, SAH, SAP

In murine infections, the inflammatory or regulatory profile of theimmune response is associated with different IgG, but such associationis less evident in humans. In Chagas heart disease (CCHD),several studies have corroborated the association of increased inflammatorycytokines levels in patients with advanced degrees ofchronic disease but the profile of subclases of IgG has been scarcelyreported. Tanking in mind that IgG1 is an inflammatory subclasswhile IgG2 and IgG4 have regulatory profiles, we hypothesized thatthese antibodies should be detected in different levels in asintomaticvs sintomatic patients. Then, we evaluated whether the severity ofmyocardiopathy is associated with an antibody subclass expressionprofile and if different antigens triggers different profiles.A cross-sectional study involving 60 individuals who underwentcomplete clinical examination was performed. Patientswere classified into three stages according to classificationproposed by Storino et al.: CCHD stage I (n = 18), CCHDstage II (n = 23), CCHD stage III (n = 19). Serum samples wereobtained for indirect ELISA assays that were performed to assessantibody levels for IgG subclasses (IgG1, IgG2 and IgG4) againsttwo mimetic antigens (P2B, B13), a constitutive one (FRA) and totalT. cruzi homogenate. For P2B, FRA and T. cruzi homogenatesthe higher reactivity was obtained with IgG2, followed by IgG1, andIgG4, respectively. For B13 the order of reactivity was IgG1> IgG2>IgG4. Along severity stages of CCHD, no significant differences inthe levels of the different subclasses were detected for any of theantigens studied (p>0.05).Although it is necessary to increase the number of patients in thesample, the homogeneity of the different antibodies subclasses levelsagainst a heterogeneous group of antigens allow to infer thatthere is no association between subclass of antibodies and degreesof heart disease in humans.