Contribution of ERK/MAPK activation to the formation of a two trial long term memory in the crab Neohelice granulata

OJEA RAMOS S; FELD M

Mar del Plata

Congreso; XXXII Congreso Anual de la Sociedad Argentina de Investigación den Neurociencias; 2017

Sociedad Argentina de Investigación den Neurociencias

Long term memory (LTM) model of the crab Neohelice granulata has been extensively studied from different points of view (e.g. behavioural, pharmacological, molecular and electophysiological). For context-specific LTM to be successfully expressed in a 24h-postraining testing session, the training protocol typically used consists of 15 trials with a 3-min intertrial interval.We study a long-term memory induced by two-trial training with an intertrial interval of 45 min. This type of training represents an attractive model for studying individual trials and intertrial interval contribution to the activation of putative molecular pathways involved in memory formation during training. It has been proposed that in two-trial training protocols the first trial induces a specific molecular context mediated by the activation of the ERK/MAPK pathway, which enables the formation of a long term memory only after a 45 min intertrial interval training. Here, we present preliminary results regarding context-specificity of this type of memory as well as synaptic ERK/MAPK pathway?s activation dynamics and dependence.