EXPRESSION OF APELIN mRNA IN THE POST-ISCHEMIC RAT KIDNEY

MOLINAS, SARA M.; ZOE SABARIZ; EMILSE WEIHMÜLLER; MARQUEZ SUSANA; LAURA TRUMPER; LILIANA A. MONASTEROLO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias de Argentina

The regulatory peptide apelin is the endogenous ligand for the orphanG protein-coupled receptor APJ. The apelinergic system hasbeen demonstrated to be involved in the pathogenesis of many conditions.Apelin treatment decreased the expression of pro-inflammatorycytokines and improved renal function in ischemia-reperfusioninjury.The aim of this work was to evaluate the expression of i) apelin,ii) toll-like receptor (TLR)-2, inductor of pro-inflammatory cytokines,and iii) heat shock protein 70 (HSP70), endogenous ligand for TLR-2, in the post-ischemic kidney.Adult male Wistar rats underwent unilateral right renal ischemiafor 40 minutes. Reperfusion was allowed for 24 hours (IR) beforekidney removal. Sham operated controls (C) were also included inthe protocol. Clearance studies and histological examination werecarried out. Apelin and TLR-2 mRNA expression was analysed byqRT-PCR; and HSP70 protein expression, by Western blot.After 24 hours of reperfusion, creatinine clearance significantlydecreased and histological observation showed multifocal acute tubularnecrosis, intratubular necrotic/apoptotic cells detachment andbrush border diminution. Apelin mRNA in the cortex of IR kidneyswas decreased (-70%, p