Epstein Barr virus (EBV) in Diffuse Large B Cell Lymphoma (DLBCL) from pediatric and adult series: a comparative study of expression and latency pattern

COHEN M; NARBAITZ M; METREBIAN F; DE MATTEO E; PRECIADO MV; CHABAY P

Oxford

Congreso; EBV 50th Anniversary Meeting; 2014

EBV Association

The 2008 WHO classification included a new entity, EBV+ DLBCL of the elderly, affecting patients ≥50 years. However, some reports of younger EBV+ DLBCL cases, without evidence of underlying immunosuppression, can be found. Type II/III latency pattern was described to be associated. Our aim was to assess EBV presence and latency pattern in an adult and pediatric DLBCL series of Argentina, to determine if there are age-specific characteristics. The study was conducted on biopsies from 74 adult and 26 pediatric DLBCL patients. EBERs expression was performed by in situ hybridization, while EBV gene expression of latent and lytic genes was analyzed by real-time PCR, and by LMP1 and LMP2A immunohistochemical expression. Nine percent of adult cases showed EBV expression, with similar frequency among patients