Enhance production of IL-10 in Regulatory Dendritic Cells induced by Trypanosoma cruzi depends on the activation of MEK/ERK pathway and is not associated to TLR2 triggering

PONCINI CV; GIMENÉZ G; PONTILLO CA; PIAZZÓN I; GONZÁLEZ CAPPA SM

Viña del Mar, Chile

Congreso; 9th Latin American Congress of Immunology; 2009

Asociación Latinoamericana de Inmunología

We demonstrated the ability of live tripomastigotes (Tp) to modulate differentiation stage and functionality of bone marrow-derived dendritic cells (DC) to a regulatory phenotype in vitro. Tp fails to activate DC, prevents fully activation by LPS, induces high IL-10 secretion and poor alloresponse. Several groups reported T.cruzi recognition by TLRs. Here we studied, by Western blot, the activation of reported key regulators of cytokine production and DC differentiation in DC cultured with live or heat-killed Tp and/or LPS. By ELISA, we analyzed cytokine production and how it was affected by the absence of certain TLRs, using DC from TLR2 KO and TLR4-mutant mice. Live and heat-killed Tp enhance ERK and induce STAT3 phosphorylation during LPS-stimulation. Tp enhances IL-10 production by LPS-treated DC and MEK inhibition leads to a reduced production of IL-10 (p