Efficacy of glass wool column filtration for stallion sperm selection and comparison with Androcoll-E? colloid centrifugation

ZEVALLOS VALENZUELA G; FERRANTE A; MARIN BRIGGILER, CI; MIRAGAYA M; VAZQUEZ LEVIN, M

Congreso; Reunión Anual de la Sociedad Argentina de Biología; 2016

EFFICACY OF GLASS WOOL COLUMN FILTRATION FOR STALLION SPERM SELECTION AND COMPARISON WITH ANDROCOLL?-E COLLOID CENTRIFUGATION Zevallos Valenzuela GE1, Ferrante A2, Marín Briggiler CI1, Miragaya M2, Vazquez-Levin MH1.1Instituto de Biología y Medicina Experimental (CONICET-FIBYME) &2Cátedra de Teriogenología (Fac de CsVet-UBA). E-mail: giulizevallos@gmail.com; mhvazl@gmail.comAndrocoll?-E colloid centrifugation is the standard technique used to select good quality stallion sperm. Among other procedures, glass wool column filtration (GWCF) has been reported to select sperm from human, bull, boar, canine and buffalo bull semen. However, there are scarce reports on its use in equine semen. We have completed a study aimed at 1) evaluating the ability of GWCF to select high quality sperm suspensions from fresh (F) and frozen-thawed (FT) stallion semen, and 2) comparing results between GWCF and Androcoll?-E colloid centrifugation procedures. In all cases, percentage of total motile (TM), progressive motile (PM), morphologically normal (MN), osmotically-competent (HOST+), and acrosome-intact + osmotically-competent (AI/HOST+) sperm were assessed before and after selection procedures. In F samples (mean±SDM; n=12), percentage of MT (72.5±14.9), MP (43±22), MN (64±12), HOST+ (57±9) were higher (p