NITRO-OLEIC ACID (NO2-OA) ACTS AS A PLANT DEFENSE SIGNAL MOLECULE IN TOMATO CELL SUSPENSIONS

ARRUEBARRENA, A.; DI FINO, LUCIANO M.; SALVATORE S; GERGOFF GUSTAVO; GARCIA-MATA, C.; SCHOPFER F; LAXALT AM

Buenos Aires

Congreso; SAIB; 2017

Nitro fatty acids (NO2-FA) act as signal molecules in mammals,through liberation of nitric oxide (NO) and/or the formation of adductswith cellular thiols (like glutathione or protein cysteine). In plants, itsformation was recently reported. In Arabidopsis, nitro-linolenic acid isproduced in response to abiotic stress. In addition, exogenousapplication increases expression of reactive oxygen species (ROS)generation/metabolisms - related genes.To have a deeper understanding of the role of nitro fatty acids on plantphysiology, we analyzed effects of exogenous application of NO2-OA on tomato cells cultures. Tomato cells cultures were treated withNO2-OA at different concentrations and as control we used oleic acid(OA). We analysed ROS and NO, plant defense related geneexpression, cell death, glutathione content and NO2-OA-protein adductformation