Heat shock protein 83 gene analysis in Leishmania genus

QUISPE RICALDE MA; LO PRESTI MS; MORALES MAS; LEHMANN RNA; VENERO RLP; PAGLINI P; MARTÍNEZ CARRETERO E; VALLADARES HERNÁNDEZ B

Sevilla

Congreso; 22nd IUBMB & 37th FEBS Congress.; 2012

Federation of European Biochemical Societies

Human leishmaniasis is a group of diseases caused by species ofthe flagellated protozoan parasites that belong to the Leishmaniagenus. There are two main clinical manifestations cutaneous andvisceral leishmaniasis. The cutaneous form affect skin and/ormucous membranes, and is characterized by the presence of singleor multiple painless lesion, nodular lesions spread over thebody, or mucocutaneous lesions that affect the nasopharyngealregion. Like other parasites, some Leishmania species coexist inthe same geographical areas, involving in some cases the samehosts, reservoirs or vectors.The gold standard identification technique is MLEE. Howeverseveral molecular markers have been described and used. Wereport here the reach of hsp83 gene for identification of Leishmaniaspecies and for the contribution in the discussion of generaltaxonomy consensus of this genus.The method used was RFLP and sequencing. Six differentenzymes were analyzed in seven species within the genus. Thewhole gene was cutted in two fragments one of 1100 pb andanother one of 944 pb, both of them were analyzed by RFLP.Moreover the fragment of 944 pb was sequenced and analyzedwith Mega software.The 1100 pb fragment has a high conserved sequence and didnot show differences between species. The 944 pb analyzed byRFLP was able to differentiate between subgenus Leishmaniaand Viannia. L. (V.) lainsoni showed a different pattern.The sequence of 944 pb of hsp83 gene, formed in Leishmaniagenus, a monophyletic group with two well differentiated subgen-era. Within subgenera Viannia, L. (V.) lainsoni form one independentcluster. L. (V.) braziliensis and L. (V.) guyanensis are closerthan L. (V.) lainsoni.