HSP83 gene analysis in species of subgenus Viannia

QUISPE RICALDE MA; POU BARRETO C; LO PRESTI MS; MARTÍNEZ CARRETERO E; PAGLINI P; PACHECO R; VALLADARES HERNÁNDEZ B

Barcelona

Congreso; 7th European Congress on Tropical Medicine and International Health; 2011

ISGlobal Barcelona. Institute for Global Health

Species of the subgenus Viannia are responsible for Americancutaneous and mucocutaneous leishmaniasis . Within the subgenusso far nine species have been described, most of which are associatedwith cutaneous lesion, variation in the disease pattern to mucosalleishmaniosis involves only L. (V.) braziliensis. On the other hand,Leishmania species coexist in the same geographic areas, makingidentification difficult at the time of application and subsequentmonitoring of treatment patients. In work done by our group haveshown the heat shock protein of 83 kDa (hsp83) gene is useful todistinguish subgenus but do not knowits capacity to identify species.The aim of this study was to analyze a fragment of the hsp83 in theirability to differentiate species within the subgenus Viannia. Theanalysis used was restriction fragment polymorphisms of 944 pbfragment of hsp83 gene. Four ATCC strains and 21 species ofLeishmania isolated from patients were used. The isolated strainswere previously characterized by RAPD and MLEE. The patterns ofdigestion with Hae II were the same to L. (V.) braziliensis,L. (V.) guyanenesis and L. (V.) panamensis, only L. (V.) lainsonipresented a different pattern. L. (V.) peruviana strain L677 on theother was different to the other two strains of the same species.Enzymes Xho I and Ksp I have the same restriction pattern for allspecies except the L677 strain that has no target for these twoenzymes. The analysis of 944 bp fragment of hsp83 gene show thephylogenetic distance of L. (V.) lainsoni with respect to other speciesof the subgenus. The results indicate the need for further comprehensiveanalysis of the hsp83 gene for possible polymorphisms. Thiswork was supported by funds provided by Agencia Espan? ola deCooperacio´ n Internacional (AECID) under the PCI program.