Pharmacokinetics of cefquinome in llamas (Llama glama) after an intravenous administration

HIMELFARB MARTÍN ALEJANDRO; LORENZUTTI AUGUSTO MATÍAS; ZARAZAGA MARÍA DEL PILAR; AGUILAR SOLEDAD; LITTERIO NICOLÁS JAVIER; BOGGIO JUAN CARLOS

Rosario

Jornada; XLI Reunión Anual de la Sociedad Argentina de Farmacología Experimental; 2009

Sociedad Argentina de Farmacología Experimental

The objective of this study is to perform a pharmacokinetic (PK) analysis of cefquinome in llamas, after an IV administration. Our experience is based in 6 adult healthy female. It has been administrated 2 mg/kg of cefquinome (Cobactan 4.5%, Intervet, Germany) in the right yugular vein. The blood samples were collected at regular intervals. Cefquinome was quantified by HPLC/uv. For the validation of the thechnique was determinate, linearity, accuracy, precision, inter-day and intra-day variation, quantification limit and recovery. This data was analyzed with PCnonlin v 4.0, and a non compartmental model was selected. The quantification limit was 0.07 mcg/ml. Pharmacokinetics parameters were: t1/2lambda 2.58; Cl 0.059; Vd 0.21 L/kg. We could agree that the developed chromatographic thechnique is valid for the purposed objectives and allows to quantify amounts of cefquinome, in serum, in the intervals of 29.33 mcg/ml and 0.07 mcg/ml. Furthermore there should be a quantification of several CIMs for local pathogens in llamas, to be able to perform a full pharmacokinetic pharmacodynamic study.