INVESTIGADORES
CABRERA KREIKER Ricardo Jorge
congresos y reuniones científicas
Título:
Allopregnanolone modifies 3 beta- HSD activity and expresión in hypothalamus and ovary of rats in an estrus cycle dependent manner
Autor/es:
GIULIANI F.; VEGA A.; CASAS S.; NANFARO F.; BAZZOCCHINI V.; YUNES R.; CABRERA, R.
Lugar:
Tandil
Reunión:
Congreso; XL Reunión Científica Anual de la de la Sociedad Argentina de Farmacología Experimental.; 2008
Institución organizadora:
SAFE
Resumen:
BV-107.
ALLOPREGNANOLONE MODIFIES 3b-HSD ACTIVITY
AND EXPRESSION IN HYPOTHALAMUS AND OVARY OF
RATS IN AN ESTRUS CYCLE DEPENDENT MANNER
Giuliani F, Vega Orozco AS, Casas S, Nanfaro F, Bazocchini V,
Yunes R, Cabrera R.
LINCE-IMBECU-CONICET. Area de Farmacología, Facultad de
Ciencias Medicas, UN de Cuyo., Ciudad Universitaria, (CP 5500)
Mendoza, Argentina. E-mail: fgiuliani@fcm.uncu.edu.ar
3-b-hydroxysteroid dehidrogenase (3-b-HSD) converts pregnenolone
to progesterone. Previously we demonstrated that
Allopregnanolone (Allo) icv inhibits the LH release, ovulation and
lutheal apoptosis. The aim was to study if Allo icv modifies enzymatic
activity and gene expression of hypothalamic and ovarian 3-
b-HSD during proestrous (P) and diestrous (D). Female Holtzman
adults rats were injected in third ventricle with Allo 6ìM or vehicle
(KRBG) and sacrified 30 min later. 3-b-HSD activity was
measured by spectrophotometric method.The gene expression was
studied by RT-PCR. The results were analized by T-test, p<0.05
was considered significant. In P, Allo decreased 3-b-HSD activity
in hypothalamus (p<0.001) and ovary (p<0.01), but no significant
changes were observed in the gene expression. In D, Allo increased
the enzimatic activity (p<0.01) and the gene expression (p<0.05)
in ovary and hypothalamus respectively. We conclude that Allo
modulates the activity and the gene expression of 3-b-HSD in hypothalamus
and ovary in an estral cycle dependent manner. Moreover
we show that the neurosteroids are important modulators of
the molecular mechanisms involved in the reproductive physiology
of the female rat.