INVESTIGADORES
FUSARI Corina Mariana
congresos y reuniones científicas
Título:
Unravelling the genes involved in plant primary metabolism regulation by Genome Wide Association
Autor/es:
FUSARI CM; KOOKE R; SULPICE R; KEURENTJES JJB; STITT M
Lugar:
Berlin
Reunión:
Workshop; Computational Life Science @Bayer Workshop; 2015
Institución organizadora:
BAYER
Resumen:
Enzymes constitute the molecular machinery for primary carbon (C) and nitrogen (N) metabolism, which provides the building blocks for growth. Previous studies in Arabidopsis thaliana populations showed that many enzyme activities correlate with each other, suggesting that their levels are under tight common regulatory control (1, 2, 3). Analysis of genetic diversity for enzyme activities offers a powerful approach to identify genes underlying the metabolism regulatory network. Few QTLs co-localising with enzyme structural genes have been identified as responsible for trait variation (2). However, trans regulation remains largely unclear.We performed Genome Wide Association (GWA) analyses on 360 Arabidopsis accessions for 21 enzyme activities and 13 metabolites from C and N primary metabolism in two independent experiments (Fig. 1). A detailed comparison between results from the two datasets was performed and SNPs with high LOD-score in both experiments were selected. GWA validation was carried out using 77 TDNA-insertion lines.We cross-mapped the coarse QTLs found in the biparental population for the structural genes UGP1 and ATBETAFRUCT4. In addition, high-LOD score SNPs were located in trans to enzyme structural genes. A total of 27 trans-regulatory QTLs were found, including a QTL associated to 4 enzyme activities, 3 metabolite levels, total protein and fresh weight. The metabolic profiling of TDNA-insertion lines confirmed and validated cis and trans-regulation for a number of genes (Fig. 2).This study provides the highest defined QTL dataset for enzyme variation to date and breaks new ground in understanding the genetic regulation of central metabolism.