NEUTROPHILS ACTIVATED BY IMMUNOCOMPLEXES MODULATE CD4 T CELL RESPONSE IN LYMPH NODES

SOFÍA DAIANA CASTELL, MARÍA FLORENCIA, GABRIEL MORÓN, BELKYS MALETTO, MARÍA CRISTINA PISTORESI

Congreso; Reunión Conjunta Sociedades de Biociencias; 2017

Previously we demonstrated that immunocomplexes (IC) generated by injecting OVA in the footpad of immunized mice that have anti-OVA antibodies, induce the migration of OVA+ neutrophil to draining popliteal lymph nodes (D-poLNs). In the present study we evaluate the influence of neutrophils activated by IC on CD4 T cell response in lymph nodes. C57BL/6 mice were immunized with OVA emulsified in Freund?s complete adjuvant and 15 days later boosted with OVA emulsified in Freund?s incomplete adjuvant. Ten days after last immunization they were injected with OVA-FITC in the footpad and D-poLNs were obtained 6 to 48 h later; saline solution was injected on footpad corresponding to control non-draining popliteal lymph nodes (ND-poLNs). OVA+ neutrophils migrated to D-poLNs 6 h after OVA injection (p