INVESTIGADORES
GALLEGO Susana Mabel
congresos y reuniones científicas
Título:
Ubiquitin-proteasome system and cellular cycle proteins in wheat under copper stress.
Autor/es:
PENA LB; AZPILLICUETA CE; PASQUINI LA; TOMARO ML; GALLEGO SM
Lugar:
Mar del Plata, Buenos Aires, Argentina
Reunión:
Congreso; XLIII Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2007
Institución organizadora:
SAIB
Resumen:
Ub-proteasome system is responsible for the degradation of proteins in
cytoplasm and nucleus, controlling the regulatory short-life proteins
that affect processes such as cellular proliferation. The aim of the
present work was to evaluate the effect of copper on Ub-proteasome
system and on proteins related to cellular cycle. Seeds of Triticum aestivum L. were floated for 48 h on distillated water (control) or adding with 1, 10 y 100 µM CuCl2.
The highest Cu concentration used inhibited the germination. Growth was
evaluated measuring the length of the primary root. While 1 µM Cu
induced the growth (15%), 10 µM Cu inhibited it (54%), effect knew as
hormesis. Membrane permeability was unchanged with 1 and 10 µM Cu
treatments. Total oxidized proteins were increased with treatments, but
total soluble and Ub-proteins contents were unaltered respect to
control. In Cu treatments, 20S proteasome protein abundance was similar
to control. Althought cyclin D protein abundance in treated seedlings
was similar to control, with 10 µM Cu decreased Ub-cyclin (30%) and
increased oxidized-cyclin level (117%) with respect to control values.
CDKD2 protein was unaffected by Cu. In conclusion, Cu did not show
effect on the proteasome system but produced oxidation of specific
proteins involved in the cellular cycle that could be responsible of
growth inhibition observed in the treated seedlings.