ATROGLIAL MGLU3R FUNCTIONS IN AGING ASTROCYTES AND HIPPOCAMPUS IN A MODEL OF ALZHEIMER DISEASE

JUAN TURATI; DELIA RAMIREZ; LILA CARNIGLIA; JULIETA SABA; CARLA CARUSO; JUAN BEAUQUIS; FLAVIA SARAVIA; DANIELA DURAND; MERCEDES LASAGA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Our laboratory recently showed that metabotropic glutamate receptors (mGlu3R) in astrocytes presents a strong neuroprotective action against different inflammatory agents like NO and Aβ. Physiological cerebral aging is characterized by an increase in oxidative stress, neuroinflammation, which among others alterations compromise cognitive functions. As an in vitro aging model, we developed primary astrocytes cultures of 3 weeks (3w) and 9 weeks (9w), treating them with themGlu3R agonist LY379268 (LY), 0.01μM/24hr. We found that the cells reach a senescence state at 9w (β-Galactosidase assay). Furthermore, we observed decreased cell viability at 9w, MTT assay. We also observed an important decrease of SOD activity comparing 9w astrocytes to 3w, while LY treatment induced a significant (p