PENETRATION OF THE ANTIBIOTIC FOSFOMYCIN INTO SWINE INTESTINAL MUCOSA COLONIZED WITH LAWSONIA INTRACELLULARIS

PÉREZ D.S., SORACI A.L., MARTÍNEZ G., FERNÁNDEZ PAGGI M.B., RICCIO M.B. DIEGUEZ S., TAPIA M.O

Nantes

Congreso; 13th International Congress of the European Association for VeterinaryPharmacology and Toxicology; 2015

European Association for VeterinaryPharmacology and Toxicology

Introduction/Objective: Fosfomycin (FOS) is an antibiotic (ATB) used for the treatment of lung and enteric infections of pigs. Intracellular fluids of enterocytes can act as biophase for Lawsonia intracellularis (LAW), causative agent of porcine proliferative enteropathy (PPE), which presence could modify ATB penetration. The aim of this study was to determine FOS penetration into swine intestinal mucosa colonized with LAW.Materials and Methods: Four healthy pigs in grow-finish stage; live attenuated vaccine (Enterisol Ileitis®, Boehringer-Ingelheim); primers for LAW PCR detection (GATAATCTACCTTCGAGACGG; TGACCTCAGTGTCAGTTATCGT, Invitrogen); calcium FOS. Explants were produced from ileum of euthanized animals. As a positive control for the PCR, LAW DNA was obtained from the vaccine. Explants (0.5 mL of vaccine; 24 h of incubation; 37ºC) were incubated with 580 µg/mL of calcium FOS (0.5 to 6 h). Then, they were washed to remove extracellular FOS, deproteinized (1 mL of methanol) and sonicated (30 min) to release intracellular ATB. Tubes were centrifuged (6 min; 4ºC; 10,000 rpm), supernatants were evaporated to dryness (60ºC), dry extracts