INVESTIGADORES
LOMBARDO Daniel Marcelo
congresos y reuniones científicas
Título:
TUNING FOR THE DETERMINATION OF RANKL AND OPG IN TEETH OF CAT BY IMMUNOCYTOCHEMISTRY
Autor/es:
SACCOMANO, DM.; GAUNA, LG.; NEGRO, VB; BOVIEZ, JD.; LOMBARDO, DM.; HERNÁNDEZ, SZ.
Lugar:
Tandil
Reunión:
Jornada; Primeras Jornadas de Ciencias Morfológicas de las Sierras de Tandil. Universidad del Centro de la Provincia de Buenos Aires (UNCPBA).; 2011
Institución organizadora:
Universidad del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias.
Resumen:
Several
studies have shown that severe periodontal disease (PD) results in dental
resorption in human and animal teeth. Although how these resorptions occur are
not entirely clear, various signals exist at the molecular level that would
trigger the responsible cells for dental destruction (osteoclasts /
odontoclasts). Nuclear Receptor Activator Complex K (RANK), its ligand (RANKL)
and osteoprotegerin (OPG) regulate the activation of cells that induce the
formation or resorption of dental tissue, according to the stimulation they
receive. No previous studies indicated that available antibodies could be used
in teeth of cat, for that the aim of this paper is to report the development of
the technique of immunocytochemistry (ICQ) for determination of RANKL and OPG in normal teeth and teeth
with periodontal disease of cat. Two normal teeth and 5 with severe EP from 3 cats
were analyzed. Teeth were fixed in buffered formaldehyde for at least 48 hours,
decalcified with 10% formic acid and processed with routine technique (H/E).
For ICQ rabbit anti goat secondary antibody
(Biotin), rabbit anti goat ,primary OPG (N-20) and RANKL (N-19) primary
polyclonal antibodies (Santa Cruz
Biotechnology®, Inc) (1/100), Dako Streptavidin
were used and revealed with DAB. Bone marrow (for RANKL) and rat small
gut (for OPG) were used as positive control. Reaction was positive in the area of the periodontium.
This study shows that these primary antibodies could be used in cat dental tissues with
positive results. Keywords: RANKL, OPG,Periodontal Disease, Immunocytochemistry, Cat.