INVESTIGADORES
ACOSTA Gabriela Beatriz
congresos y reuniones científicas
Título:
Comparation between acute and chronic postnatal stress in glutamatergic neurons in the rat brain
Autor/es:
MARIA MERCEDES ODEON; ADRIAN E SALATINO; MARIA LUZ ORTA; GABRIELA ACOSTA
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; First Joint Meeting of the Argentine Society for Neurosciences (SAN) and the Argentine Workshop in Neurosciences (TAN); 2009
Institución organizadora:
SAN y Taller de Neurociencias
Resumen:
Comparation between acute and chronic postnatal stress on glutamatergic neurons in the rat
Odeón María Mercedes, Salatino Adrián Emanuel, Orta María Luz, Acosta Gabriela Beatriz
Instituto de Investigaciones Farmacológicas (ININFA) (CONICET-UBA). Junín
956. 5th floor, C1113AAD, Buenos Aires, Argentina
E-mail: merodeon@hotmail.com
It is well known that animals exposed to stressful stimuli during their
early life develop different neurological disorders when they become adults.
In this study, we evaluated the consequences of acute and chronic stress on
adult brain on Glutamate transporter (GluT) in vitro, evaluating the uptake
of [3H]-Glu by synaptosomes-enriched fractions isolated from rat cerebral
frontal cortex (FC) and hippocampus (HIC) by time-course and kinetic
studies. In acute stress, the rats were separated from their mothers and
divided in two groups: control and stress. While control rats were moved to
a separated cage, stress rats were exposed to cold stress (4ºC) during 1 h.
In repeated stress the rats were separated from their mothers and exposed
to cold stress (4ºC) for 1 h at postnatal day (PD) 7 during 20 days. These
animals were allowed to a 30 days recovery period until adulthood. At the
end of the stress period, animals were killed by decapitation. FC and HIC
were dissected to study GluT and trunk blood samples were collected to
determinate corticosterone levels. Acute stress results show an HIC increase
in both affinity (Km) and maximum velocity (Vmax), while in the FC only
the affinity was increased. Repeated chronic stress shows changes only in
Vmax: rise in FC and decrease in HIC. The time-course on FC in acute stress
shows an uptake decrease while in chronic stress the uptake was similar
in both groups. HIC did not show significant differences either chronic or
acute stress. The levels of corticosterone decreased in chronic stress. These
results suggest that the exposure to early stressful adverse life events affects
hypothalamic-pituitary-adrenal (HPA) axis and alter the glutamatergic
neurons.