Effect of glutamine on the alteration in Na+, K+ ATPase induced by nephrotoxic doses of acetaminophen (APAP) in rats

MARCO BOVEDAN; MOLINAS, SARA M.; PISANI, GERARDO B.; MONASTEROLO, LILIANA A.; LAURA TRUMPER

Rosario

Congreso; XVIII Congreso y XXXVI Reunión Anual de la Sociedad de Biología de Rosario; 2016

Sociedad de Biología de Rosario

We have previously reported the development of acute renal failure after the administration of a toxic dose of APAP. A nephrotoxic dose of APAP promoted an increased level of Na+, K+ ATPase and its detachment from its membrane anchoring as well as functional and histological alterations. HSP70 has been associated with cytoprotection in response to several injuries. Our aim was to study the effects of glutamine (GLN) on the alterations in Na+, K+ ATPase promoted by APAP. Male Wistar rats were used. Treatments: Administration of i) single dose of APAP 0.75 g/kg b.w. i.p (APAP, n=6). ii) single dose of GLN 0.75 mg/kg b.w.. i.p (GLN, n= 4) iii) GLN 30 min before APAP (GLN/APAP n = 5). iv) vehicles (Control, n= 5). At the end of a 16 h-collection period, animals were anesthetized and blood and kidneys were collected. Renal function, HSP70 cortical abundance and Na+, K+ ATPase cortical abundance and distribution were analysed. GLN protected against the renal functional effects of APAP. HSP70 expression was induced in APAP, GLN and GLN/APAP groups. Na+, K+ ATPase abundance was increased to the same level in the APAP and the GLN/APAP group, but Na+, K+ ATPase maintained its basolateral localization in rats receiving the dual treatment. Our results support the idea that GLN pretreatment could be a therapeutic option to prevent APAP-induced renal injury