Evidence that inhibition of DHEA production by adrenal cells treated with culture supernatants from M tuberculosis-stimulated PBMC is abrogated upon TGF-ß neutralization.

BAY ML; D'ATTILIO L; GORDILLO M; BOTTASSO O

Congreso; V Reunión Sociedad Latinoamericana de Tuberculosis y Otras Micobacteriosis; 2010

Evidence that inhibition of DHEA production by adrenal cells treated with culture supernatants from M tuberculosis-stimulated PBMC is abrogated upon TGFβ neutralization.Bozza V1, D?Attilio L1, Gordillo M1, Alfonso J1, Dídoli G1, Besedovsky H2, del Rey A2, Bottasso O1, Bay ML1.1Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Argentina; 2 Institut für Physiologie und Pathophysiologie, 35037 Marburg, GermanyPreviously we demonstrated that pooled culture supernatants from PBMC (SN) of TB patients inhibited DHEA secretion by the human adrenal cell line NCI-H295-R. As well as being a major cytokine produced by M tuberculosis (Mtb)-stimulated PBMC, TGFβ is likely to be implied in this regard. To address this issue, SN from PBMC cultured during 24, 36 or 96 h, undergoing Mtb stimulation (SNMtb) or not (SNBasal) of healthy controls (HCo, n=10) or severe TB patients (Sev, n=7) were assayed for their effects on steroid production by adrenal cells. Cortisol and DHEA were assessed in fluids from 48 h-cultured NCI-H295-R cells, activated or not with Foskolin (Fk), without or with anti-TGFβ (at 25:1, 10:1 or 1:1 ratio; according to average concentrations of TGFβ in SNMtb: 6.1 ng/ml). Treatment with 36 h-cultured SNMtb-Sev inhibited DHEA production by adrenal cells (p