In vivo effect of endocrine disruptors (etanol and endosulfan) on chromatin decondensation in roden species

SANCHEZ M; ROMANATO M; MILESI M; ALARCÓN R; CEBRAL E; LO NOSTRO F; LUQUE E; CALVO JC

Chascomus

Congreso; XVI Jornadas Anuales de la SAB; 2014

Sociedad Argentina de Biología

Chromatindecondensation is the first step towards syngamy after the spermatozoonpenetrates the oocyte. It involves protamine disulfide bond reduction as wellas protamine - oocyte histone exchange with the aid of a negatively chargedmolecule, s. Ethanol and endosulfan are known for their endocrine disrupting effectson gonads. We have previously reported that endosulfan, increases mouse spermchromatin decondensation in vitro.Here we studied the in vivo effect ofboth ethanol and endosulfan on male mice and rats, respectively. Male mice(60-90 days old) were fed with 15% ethanol in drinking water, for 15 days, andthen euthanized. Male rats were injected with 0.6 mg/kg body weight ofendosulfan in mineral oil at day 1, 3, 5 and 7 after birth and euthanized atday 60.  Spermatozoa were obtained from thecauda epididymis and decondensation analyzed in the presence of glutathion andheparine/dermatan sulfate. While mouse spermatozoa decondensed after a 60minute incubation, rat spermatozoa needed 17 hours in order to achieve asimilar degree of decondensation. Ethanol significantly increased (48+3% control, n=8 versus 57+4 % treated, n=10, p<0.05) chromatindecondensation. Conversely, endosulfan treatment completely abolished spermchromatin decondensation in rats (36+12 % in controls). These results suggestthat different endocrine disruptors could be differentially affecting eachanimal species.