Proline hydroxylation & O-arabinosylation on extensins: key post-translational modifications in root hair growth.

VELÁSQUEZ SM; MARZOL E.; MANGANO S; DENITA JUAREZ SP

Buenos Aires

Congreso; Glycoar 2014; 2014

INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES (IIBBA) ; (CONICET - FUNDACION INSTITUTO LELOIR)

Plant cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins including highly O-glycosylated extensins(EXTs) proteins. Our group has characterized several post-translational modifications (PMTs) of EXTs in the root hair cell in Arabidopsisthaliana. The root hair is a single cell with polarized growth and it is responsible for the root?s water and nutrient uptake. Previously, wehad demostrated that O-glycosylation on EXTs is essential for cell wall self-asembly and hence, root hair elongation. We have determinedseveral molecular playes involved in proline hydroxylation (Prolyl-4-hydroxylases 2,5 and 13 and subsequent O-arabinosylation(arabinosyltransferases RRA3 and XEG113-2) in EXTs.