Immune response triggered by a novel vaccine against pertussis

GRISELDA MORENO; EMILIA GAILLARD; DAVID SABATER MARTÍNEZ; EUGENIA ZURITA; DANIELA BOTTERO; AGUSTINA ERREA; ERIKA BARTEL; SOL BRAVO; FRANCISCO CARRIQUIRIBORDE; CELINA CASTUMA; MARTIN RUMBO; DANIELA HOZBOR.

Buenos Aires

Congreso; 1st LASID-SAI-FAIC Meeting; 2015

Pertussis remains a public health problem despite the availability of classical whole-cell vaccines (wP) and cellular vaccines (aP). Increased pertussis cases were detected in countries including those with high-coverage vaccine. This increment was attributed to waning immunity and pathogen adaptation. Regarding waning immunity was recently estimated that the average duration of vaccine using a calendar that contains aP doses is ? 3 years, assuming 85% vaccine efficacy. Individuals vaccinated with wP or aP boosters, the duration of immunity was higher. This could be due to different immune responses induced by two types of vaccines. While the wP promotes Th1/Th17 responses, aP induces strong Th2/antibody response with low Th1/Th17 contribution.Recently we disgned a new vaccine based on outer membrane vesicles (OMVs) derives from Bordetella pertussis, wich behaved in animal model as vaccine candidate. We evaluated humoral and cell-mediated immune responses induced by OMVs. Significant amounts of anti-Bp antibodies were detected in OMVs-vaccine immunized mice with low ratio of IgG1/IgG2a. Regarding cellular response, we detected IFNg in the supernatant of OMVs-stimulated spleen cells from OMVs ( 1,000 ± 200pg/ml) and wP-immunized mice (5,000±850pg/ml). Weak response was observed in aP-immunized mice (50±15pg/ml). In contrast IL-5 secretion was mainly detected in spleen cells from aP mice (1500±250pg/ml). High levels of IL-17 secretion were observed in OMV and wP mice (1500±300 pg/ml and 2500±450 pg/ml respectively) respect to aP mice ( 200±50 pg/ml). All this results showed that OMVs-vaccines elicits a Th1/Th2 and Th17 immune response profile and induces robust antibody response.