SHORT CHAIN FATTY ACIDS PRODUCT OF MICROBIAL FERMENTATION MODULATE MYELOID CELL ACTIVATION IN VITRO

ERREA, A ; IRAPORDA, CAROLINA; CAYET, DELPHINE; ABRAHAM, ANALÍA G.; GARROTE, GRACIELA; SIRARD, JEAN CLAUDE; RUMBO, MARTÍN

Mar del Plata

Congreso; LXII REUNIÓN ANUAL Sociedad Argentina de Inmunología-LIX REUNIÓN CIENTÍFICA ANUAL Sociedad Argentina de Investigación Clínica; 2014

Mucosal immune response may be modulated by microbial metabolites.The recent discovery of several signaling receptors ableto sense short chain fatty acids (SCFA) have placed these moleculesas putative mediators of host-microbial interaction, instead ofconsidering them as mere inert terminal metabolic products. SCFAare present in high levels in large intestinal lumen where theyare produced by intestinal microbiota. The aim of this work wasto determine if SCFA (butyrate, propionate, acetate and lactate)can modulate the properties of activated bone marrow derivedmacrophages (BMMs) or dendritic cells (BMDCs). C57BL/6 wasused as bone marrow source. BMMs were derived upon 9 daysdifferentiation in presence of M-CSF and BMDCs were derivedupon 9 days of differentiation in presence of GM-CSF. Cells werestimulated with 100 ng/mL of E.coli LPS, in presence of different concentrations of the different SCFA. Activation of BMDCs wasmonitored by changes in surface expression of CD40, CD80 andCD86 and IL6 and IL12 production upon overnight incubation.Secretion of IL6 and IL12 was evaluated as indicator of BMM stimulation.In all cases SCFA modulated surface expression of CD40in BMDCs (p