A B-CELL SUPERANTIGEN INDUCES THE APOPTOSIS OF MURINE AND HUMAN MALIGNANT B CELLS

DUARTE ALEJANDRA; LORENZO DANIELA; MUNDIÑANO JULIANA; BERGUER PAULA; SIELECKI ALEJANDRO; ALEMAN MERCEDES; NEPOMNASCHY IRENE; PIAZZON ISABEL

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAI, SAIC, SAFE

B-cell superantigens (Sags) are bacterial and viral proteins which affect immune responsiveness binding conserved sites of the VH or VL regions of immunoglobulin molecules outside their complementarity-determining regions inducing apoptosis of normal cognate B cells. We had demonstrated that T-cell Sags induce the apoptosis of cognate malignant T cells.Exposure to bacterial and retroviral T Sags in vivo increases lymphoma-bearing mice survival. In the present study we investigated whether protein L (PpL), a B-cell Sag that interacts with κ+ bearing cells are able to induce the apoptosis in spontaneous murine lymphoma B cells and human Daudi cells. Results: PpL induced apoptosis (measured by Annexin V-FITC) in murine κ+lymphoma B both in vitro (p