INVESTIGADORES
AMODEO Gabriela
congresos y reuniones científicas
Título:
The water to solute permeability ratio governs the osmotic volume dynamics in beetroot vacuoles
Autor/es:
VICTORIA VITALI; MOIRA SUTKA; OSVALDO CHARA; AMODEO, GABRIELA; MARCELO OZU
Lugar:
Tucuman
Reunión:
Congreso; III Latin American Federation of Biophysical Societies (LAFeBS) ? IX IberoAmerican Congress of Biophysics ? XLV Reunion Anual SAB; 2016
Institución organizadora:
Sociedad Argentina de Biofisica
Resumen:
arPlantcellvacuolesoccupyupto90%ofthecellvolumeandareconstantlysubjectedtowaterandsoluteexchange.Theosmoticflowandvacuolevolumedynamicsreliesonthevacuolemembrane-thetonoplast-anditscapacitytoregulateitspermeabilitytobothwaterandsolutes.Theosmoticpermeabilitycoefficient(Pf)istheparameterthatbettercharacterizesthewatertransportwhensubmittedtoanosmoticgradient.Usually,Pfdeterminationsaremadeinvitrofromtheinitialrateofvolumechange,whenafast(almostinstantaneous)osmolalitychangeoccurs.Whenaquaporinsarepresent,itisacceptedthatinitialvolumechangesareonlyduetowatermovements.However,inlivingcellsosmoticchangesarenotnecessarilyabruptbutgraduallyimposed.Undertheseconditions,waterfluxmightnotbetheonlyrelevantdrivingforceshapingthevacuolevolumeresponse.Inthisstudy,wequantitativelyinvestigatedvolumedynamicsofisolatedBetavulgarisrootvacuolesunderprogressivelyappliedosmoticgradientsatdifferentpH,aconditionthatmodifiesthetonoplastPf.Wefollowedthevacuolevolumechangeswhilesimultaneouslydeterminingtheexternalosmolalitytime-coursesandanalyzingthesedatawithmathematicalmodelling.
