Extraction and evaluation of larvicidal effect of essential oils from Eucalyptus hybrids on Aedes aegypti (Diptera: Culicidae)

LUCÍA, A; MASUH, H; LICASTRO, S & ZERBA, E.

Detroit, Michigan. USA

Congreso; 72nd Annual AMCA Meeting and 16 th Latin American Symposium; 2006

American Mosquito Control Association - USA

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES-AR; mso-no-proof:yes;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> In the search for new control tools against Ae.aegypti, larvicidal effect of essential oils from five species of Eucalyptus (myrtaceae) (E.camaldulensis; E.tereticornis, E.grandis and their hybrids E. grandis x camaldulensis y E. grandis x tereticornis ) were evaluated. Oils were obtained by hydrodistillation of vegetal material collected in an experimental plot of our Centre in Buenos Aires, Argentina (S34 33.223, W58 30.523). They were used in acetonic solution for testing and third or early fourth stage Ae.aegypti susceptible  CIPEIN strain larvae were used as biologic material. Mortality was determined after 24 hours. The results showed that Eucalyptus oils containning alfa-pinene as their main component have lower CL50 values than those that have 1,8-Cineol as the major component. Pure alfa and beta pinene produced the lowest CL50 values, ranging from  12.1 ppm to 15.4  ppm, while 1,8-Cineol had a CL50 of 57.2 ppm. By GC-MS analysis, important cuali-cuantitative differences were seen in the essential oil composition of the hybrids in comparison with their parents. 1,8-Cineole and alfa-pinene were the main components. Yields obtained from hybrids individuals were almost two times greater than those obtained from their parents. These results suggest the potential use of the controlled crossbreeding technique to obtain interespecific hybrids individuals with enhanced yield and better larvicidal effect of their essential oils against Ae.aegypti.