The presence of prokaryotic RNA in live bacteria is necessary to generate bactericidal responses in neutrophils

RODRIGUEZ-RODRIGUES, NAHUEL; MARTIRE GRECO, DAIANA; CASTILLO, LUIS A.; LANDONI, VERONICA I.; MILILLO, M. AYELÉN; SCHIERLOH, PABLO; BARRIONUEVO, PAULA; FERNABDEZ, GABRIELA C.

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC) LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) XLVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016

While PAMPs are present in live and dead bacteria, vaccines developed from live microorganisms generate much vigorous immune responses, suggesting the existence of certain determinants related to bacterial viability that enhance immunity. Because neutrophils (PMN) are the first line of defense against infections, our aim was to determine if PMN have the ability to recognize bacterial viability. Different functions of PMN isolated from healthy donors were evaluated against live E. coli (EC) and heat-killed E. coli (HKEC) (ratio PMN:EC=1:20). We noted that the responses of PMN against EC were higher compared to HKEC in all evaluated functions: generation of ROS by FACS (%DHR PMN: EC=32.8±3.9;HKEC=9.9±3.8, p