Sequencing Analysis of Fusobacteria in 3 Sites to Identify Genealogy

JAMILA TAYLOR; JIHUI REN; ROXANA RUBINSTEIN; RICHARD FRIEDMAN; YIPING HAN

San Fransisco

Conferencia; 2017 IADR/AADR/CADR; 2017

International Association for Dental Research

Objectives: The goal of this project is to compare the 16srRNA gene sequences of F. nucleatum detected in the oral cavity of patients with IBD to those detected in the stomach aspirates and stool samples from the same patients. We hypothesize that the Fn detected in the stomach and stool will be the same Fn as those present in the saliva.Methods: DNA was extracted from the oral, stomach and stool samples, followed by PCR amplification of the 16s rRNA genes using Fn specific primers (785F and BW-R1). The amplicons were then cloned and sequenced (using Sanger sequencing). Fn sequences were compared within and between patients.Results: We have analyzed 6 patients and compared the Fn present in the mouth, stomach and stool. We have used unweighted unifrac p-values associated with the Fn 16S rRNA sequences to estimate whether the communities are significantly different. The preliminary data suggests that bacteria from the three samples sites within the same patient are similar, but bacteria from the various patients are different.Conclusions: Preliminary data indicates translocation of Fn from the oral cavity to the gut. A larger sample size will bring more power to our analyses, so further work on this project is warranted. The idea that oral bacteria with virulent properties may have the ability to escape the oral cavity and play a role in disease progression at other sites throughout the body can have far-reaching implications if future work supports this claim.