Anticancer activity of lipidic nanoparticles containing essential oils components in hepatocarcinoma cells

RODENAK-KLADNIEW B; DE BRAVO MG; ISLAN GA; DURAN N; NOACCO N; CASTRO GR

Conferencia; Internation Conference on Current Trends in Biotechnology; 2016

Human hepatocellular carcinoma (HCC) is one of the most common and lethal cancers worldwide andcurrent therapies are often limited. New pharmacological alternatives are focus in the use of plant-derivedcompounds showing anticancer activities. To improve their bioavailability, solid lipid nanoparticles (SLN)arise as a potent tool for improving their efficacy in physiological environments. In the present work,linalool (LN) -a plant-derived monoterpene- was loaded into SLNs and the anticancer potential wasevaluated in HCC HepG2 cells. SLNs composed of myristyl myristate were synthesized by ultrasonicationmethod and LN encapsulation efficiency around 90% was obtained. An in vitro controlled released wasobserved for 72 h. The nanoparticles exhibited spherical size and a mean diameter of 92.3 ±0.7 nm withlow dispersion (PDI 0.21) determined by dynamic light scattering (DLS) and transmission electronmicroscopy (TEM). The loaded nanoparticles were extensively characterized by FTIR, XRD, DSC andTGA, indicating a clear nanostructuration of the lipidic matrix after LN incorporation. The formulationshowed cytotoxic activity against HepG2 cells in a dose/time manner, enhancing the anticancer activity offree LN. In addition, the cellular uptake of fluorescent-labeled SLN revealed the ability of nanoparticles toenter into cells and intracellularly deliver the cargo molecules.