INVESTIGADORES
PICOLLO Maria Ines
congresos y reuniones científicas
Título:
Adulticidal and ovicidal effect of experimental lotions based on Ivermectin
Autor/es:
CLAUDIA VASSENA, ANABELLA GALLARDO, PAOLA GONZÁLEZ AUDINO, GASTÓN MOUGABURE CUETO, ARIEL TOLOZA & MARIA I. PICOLLO
Lugar:
Urgup, Cappadocia
Reunión:
Congreso; 9. Fourth International Conference on Phthiraptera (ICP4); 2010
Institución organizadora:
International Society of Phthirapterists
Resumen:
The high levels of pyrethroid
resistance in the majority of the lice populations in Buenos Aires, Argentina, challenges us to assess
pediculicidal activity of Ivermectin, a drug used for a variety of parasitic
diseases due to its high activity and its safety on humans. We investigated the adulticidal and ovicidal activity of Ivermectin
technical and experimental hidro-alcoholic lotions based on this insecticide against permethrin-resistant
head lice. Children aged 3-13 were examined for head lice, which were removed by
a fine-toothed metal comb. After collection, lice were maintained in an environmental chamber at 18
± 0.5 °C and 70- 80% RH in the dark
for a maximum of one hour before their use in toxicological bioassays.
Insecticidal
activity was assessed by topical application on adults and nits. The Lethal Dose
50% for Ivermectin and permethrin showed that Ivermectin was 60 times more
effective than permethrin on both adults and nits.
The effectiveness of
experimental lotions was evaluated by the immersion method. Batches of 10 adults were submerged for 2
minutes in 1 ml of insecticide lotions, and washed with 100 ml of water.
Mortality was recorded after 18 h. The ovicidal activity of lotions was
evaluated by 10 min immersion of batches of 10 eggs fixed to glass slide, washed
in 50 ml water and dried over filter paper. Treated eggs were maintained at
28°C- 75 % RH until hatching of untreated eggs.
The
Lethal Concentration 50% showed that the lotions with ivermectin are more
effective than those with permethrin on both adults and nits, indicating that are
a good alternative for commercial head lice products.