MESENCHYMAL STROMAL CELLS ARE MODULATED BY A THYROID HORMONE-RESPONSIVE TU- MOR MICROENVIRONMENT

MARIANA ANDREA AMORÓS; MARÍA FLORENCIA CAYROL; LUCIANA MARIEL GUTIÉRREZ; LEANDRO CERCHIETTI ; ALEJANDRO CORREA DOMINGUEZ; CARLOS LUZZANI; SANTIAGO G. MIRIUKA; GRACIELA CREMASCHI; MARCELA FABIANA BOLONTRADE

Mar del Plata

Congreso; SAIC SAE SAFE 2016; 2016

SAIC

esenchymal Stromal Cells SC are recruited and home in the tumor stroma. Functional properties such as immuno- modulation, migration and secretion of paracrine factors turn SC as modulators of the tumor environment. dentifying the niche components that modulate the cross tal bet een tumor cells and SC is ey to understand progression mechanisms. Tumor environmental factors may affect SC functions, in uencing their tumor modulation ability ith an impact in tumor progression. n this regard thyroid hormones TH affect tumor gro th. n order to study the contribution of SC to tumor development e used our previously established model for T cell lymphoblastic leu emia ith SCs as recruited tumor stromal cell components, evaluating the modulation exerted by TH on SC in a tumor environment context. e demonstrated that TH modulated tumor cells canoni- cal path ay produced a secretome that induced lo er migration rates and higher adhesiveness, together ith an increased ability to induce angiogenesis in vitro and a higher proliferation rate on SC. hen tumor cells ere modulated by TH via surface recep- tors non canonical path ay the resultant secretome induced higher migration rates , , vs , , o. of cells/ eld, non canonical vs canonical respectively, p . , lo er adhesiveness, lo er ability to induce angiogenesis , , vs , o. of branches/ eld, non canonical vs canonical respec- tively, p . , and lo er proliferation rates on SC, suggesting a differential mechanism of action. TH also directly affects SC inducing a differential proteomic pro le on the secretome produced by TH stimulated SC. ur data suggest that tumor recruited SC articulate the effect of TH on tumor cells, being modulated and simultaneously modifying the tumor environment. y discerning the mechanisms that govern SC/tumor cells cross tal e could contribute to elucidate underlying mechanisms involved in tumor stroma transformation and progression.