Effects of heme oxygenae induction on oxidative stress markers in the liver of insulin resistant rats

WISZNIEWSKI, M; VECINO, CV; CALANNI JS; SANCHEZ PUCH, S; CYMERYNG, CB; REPETTO, EM

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016

Sociedad Argentina de Investigación Clínica

Insulin resistance (IR) is a key factor involved in thepathogenesis of non alcoholic fatty liver disease alongwith obesity and type 2 diabetes. As heme oxygenase-1(HO-1) has been recognized as an antioxidant enzymeplaying a role in cellular defense mechanisms the aim ofthis study was to examine the effects of pharmacologicalmanipulation of HO-1 on cytoprotective systems in theliver of IR rats. Male Wistar rats were randomly distributedin different groups: control (C), sucrose-rich diet (SRD,30% sucrose in the drinking water over 12 weeks). Hemintreatment (15 mg/kg/48h, ip) was initiated after 10weeks of diet (H and SRD+H groups) and continued for2 weeks. Insulin sensibility was evaluated with an insulintolerance test. Serum samples were obtained for glucoseand triglyceride (TG) determination as well as liver tissuefor the analysis of superoxide dismutase (SOD) and catalase(CAT) activities. Results indicate that administrationof SRD (SRD and SRD+H) correlates with a decrease ininsulin sensitivity. No differences in glycaemia or bodyweight were observed due to pharmacological or dietarytreatment vs C. TG levels were increased only in SRDgroup vs C (p