Intramyocardial treatment with high doses of High mobility group box-1 (HMGB1) protein improves cardiac function in sheep with myocardial infarction

BAUZÁ MARIA DEL ROSARIO; LOCATELLI PAOLA; GIMÉNEZ CARLOS SEBASTIÁN; DE LORENZI ANDREA; WRAY SANDRA; CUNIBERTI LUIS; CROTTOGINI ALBERTO; OLEA FERNANDA DANIELA

Mar del Plata

Congreso; Congreso de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

Introduction: The effect of the pro-inflammatory HMGB1 protein on experimental infarct size is controversial. A possible reason is the lack of knowledge about the therapeutic doses. In a previous study on sheep with acute myocardial infarction (AMI) comparing 1 ug and 10 ug injected intramyocardially, we have shown that only the 10 ug dose induces angio-arteriogenesis and overexpression of genes involved in cell differentiation. We now aimed at evaluating the effect of this dose on left ventricular function in the ovine AMI model.Methods: Four hours after coronary ligation, sheep were randomized to receive intramyocardial HMGB1 10 ug or PBS (placebo) in the peri-infarct zone. Echocardiographic assessment of percent wall thickening of the peri-infarct septum (%SWT) and the peri-infarct antero-lateral wall (%AWTh), and percent ejection fraction (%EF) was performed at baseline, 3 and 30 days post-AMI. Statistics: two-way ANOVA-Bonferroni; data expressed as X±SE; significance: p