IBIMOL   23987
INSTITUTO DE BIOQUIMICA Y MEDICINA MOLECULAR PROFESOR ALBERTO BOVERIS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Interactions of large procyanidins with membranes and the regulation of cell signaling
Autor/es:
M. DA SILVA; G. JAGGERS; A. G. ERLEJMAN; C. G. FRAGA; P. I. OTEIZA
Lugar:
Santa Barbara, EEUU
Reunión:
Congreso; Oxygen Club of California 2008 Meeting; 2008
Institución organizadora:
Oxygen Club of California
Resumen:
We have presented evidence that large procyanidins (LP) can interact
with membranes and protect intestinal cells from different
pro-inflammatory stimuli. We have now tested if LP could prevent
the triggering of different events that has been associated to lipid
rafts, i.e calcium mobilization, oxidant formation, and activation of
select cell signals. To test this hypothesis, we used liposomes, and
Caco-2 cells as a model of intestinal epithelium, and investigated
the effects of a fraction enriched in LP isolated from cocoa. LP
prevented Triton-X 100-mediated disruption of synthetic liposomes
enriched with glycoplipids (main components of lipid rafts).
In Caco-2 cells, LP inhibited NF-êB activation initiated by different
pro-stimulatory compounds, showing the highest inhibitory capacity
for signaling initiated at lipid rafts (e.g. tumor necrosis factor
alpha). Furthermore, LP inhibited deoxycholate-induced calcium
mobilization, oxidant production, and activation of mitogenactivated
kinases (MAPKs). In summary, LP can interact with synthetic
and biological membranes and protect them from different
pro-inflammatory stimuli. The obtained results suggest that LP
could have a relative selectivity to interact with particular areas of
the membrane, e.g. lipid rafts, and to modulate oxidant and signaling
events initiated in these areas.êB activation initiated by different
pro-stimulatory compounds, showing the highest inhibitory capacity
for signaling initiated at lipid rafts (e.g. tumor necrosis factor
alpha). Furthermore, LP inhibited deoxycholate-induced calcium
mobilization, oxidant production, and activation of mitogenactivated
kinases (MAPKs). In summary, LP can interact with synthetic
and biological membranes and protect them from different
pro-inflammatory stimuli. The obtained results suggest that LP
could have a relative selectivity to interact with particular areas of
the membrane, e.g. lipid rafts, and to modulate oxidant and signaling
events initiated in these areas.