EGT protocols: the role of electroporation based techniques, hyaluronidase and pH effects in the permeabilization of tissue fibers

OLAIZ NAHUEL; SIGNORI EMANUELLA; MAGLIETTI FELIPE; SOBA ALEJANDRO; GUERRA LILIANA; SUÁREZ CECILIA; COLOMBO LUCAS; TURJANSKI PABLO; MICHINSKI SEBASTIÁN; MARSHALL GUILLERMO

Bochum

Simposio; COST Symposium on Electroporation and Biomedical Applications and MC Meeting; 2013

COST (European Cooperation in Science and Technology)

It is known that plasmid gene transfer efficiency in tissue fibers such as muscle can be significantly improved by the application of an electrotransfer based technique to the muscle following injection of bovine hyaluronidase and plasmid DNA. Despite a less damage in muscle fibers due to the pre-treatment by hyaluronidase, traces of necrosis still remain visible in the tissue with a concomitant loss of transfected tissue fibers and reduction in expression. Here we analyze with experimental measurements and theoretical modeling different factors producing tissue necrosis such as electric pulse parameters, electrode geometry as well as possible pH effects. Ways to optimize the EGT protocol are suggested.