INVESTIGADORES
SUAREZ Cecilia Ana
congresos y reuniones científicas
Título:
ph changes induced by EGT and ECT pulse delivery in tissues
Autor/es:
MAGLIETTI FELIPE; MICHINSKI SEBASTIÁN; OLAIZ NAHUEL; CASTRO MARCELO; SUÁREZ CECILIA; MARSHALL GUILLERMO
Lugar:
Barga
Reunión:
Congreso; Gordon Research Conference in Bioelectrochemistry; 2012
Institución organizadora:
Gordon Research Conferences
Resumen:
Experimental
evidence of the existence of pH fronts emerging from both electrodes
during tissue reversible electroporation for conditions typical to
many clinical studies found in the literature, is presented. It was
also observed that those fronts are immediate and substantial. pH
fronts are indirectly measured through the evanescence time, that
is, the time taken by the tissue buffer to neutralize them. In
order to compute the evanescence time, the evolution of a pH
indicator was imaged at a series of time points, and a four-cluster
fuzzy-c-means method was used to segment the pixels corresponding to
the pH indicator at every frame. The evanescence time was
computed as the time when the number of pixels was 10% of that in the
initial frame. While in reversible electroporation (ECT)
evanescence is very fast due to biological buffers present in the
tissue (less than 10 s, though enough to cause minor injuries),
in electrogenetransfer (EGT), evanescence is much slower and enough
to denaturate plasmids. Remarkably, when any of the pulse
parameters in EGT is reduced by one half, the evanescence time drops
significantly. This has relevant implications for EGT treatment
efficiency, since much less plasmid damaging occurs.