Adh1 first intron polymorphism in wild Argentinean population of Ceratitis capitata

SILVIA LANZAVECCHIA; NATALIA PETIT-MARTY; NATALIA PACIENZA; GUSTAVO YANNARELLI; DIEGO F. SEGURA; JORGE L. CLADERA; RUBEN ZANDOMENI

Mendoza

Congreso; 4th Meeting of the Working Group on Fruit Flies of the Western Hemisphere; 2001

Populations of Ceratitis capitata from Argentina were characterized studying the first intron of the alcohol dehydrogenase 1(Adh1) gene. The flies were sampled by fresh fruit recollections from the main producing areas of the country. The DNA was obtained in individual extractions from adult flies. The different sampled sites were grouped according to its geographical location defining 19 populations for genetics studies. Using EPIC-PCR (Gomulski et al, 1998)a  we studied 246 individuals detecting three size variants (2700, 2600 and 2000 bp) of Adh1 first intron. Further digestion with EcoRI allows a better differentiation of the alleles. Population allelic frequency were analyzed obtaining a total heterozygosity (He)=0.34. In most populations, the observed and expected allelic frequencies are conformed to Hardy-Weinberg expectations (p>0.05) according to the “Exact Test.” Significant differences between some populations were found using the “Population Pairwise Differentiation Test”. Also, we obtained the complete sequence of the three-allele variants allowing the analysis at the nucleotide level. The population variability data and the sequence of this noncoding region of ADH1 locus, will allow to evaluate the allelic variants as differential markers, in order to characterize natural argentinean populations of C. capitata.