Brain and gonadal gene expression profile in juvenile pejerrey (Odontesthes bonariensis) exposed at 17alfa-ethinylestradiol

PÉREZ, MR; FERNANDINO, JI; BLASCO, M; CARRIQUIRIBORDE, P; SOMOZA, GM

Rouen

Simposio; Satellite Symposium: Neuroendocrine effects of endocrine disrupters. 7th International Congress of Neuroendocrinology.; 2010

Olivier Kah, Vance Trudeau (Chairs)

Brain and gonadal gene expression profile in juvenile pejerrey (Odontesthes bonariensis) exposed to 17alfa-ethinylestradiol. The aquatic environments subjected to poorly treated sewage discharges present a wide variety of chemical contaminants that potentially interfere with the endocrine system. In this context, the aim of this study was to evaluate the effect of 17alfa-ethinylestradiol (EE2) on gene expression of key gonadal steroidogenic enzymes and brain aromatase of pejerrey fish. Juvenile males were exposed for 10 days to 15 ng/L EE2, a concentration presented in water bodies where pejerrey inhabits. Despite the fact that no differences were observed at gonadal morphological level, EE2 treatment inhibited the cyp11b2 and 11beta-HSD, and stimulated the cyp19a1 in gonads, and cyp19a1b in the brain at the mRNA level. These results suggest the beginning of a process of gonadal feminization, and then the expression of these genes could be used as biomarker for endocrine disruption studies.