INVESTIGADORES
MARTINEZ TOSAR Leandro Julian
congresos y reuniones científicas
Título:
A putative role for the double-stranded RNA-binding protein Staufen 1 in stress granules´ physiology
Autor/es:
MARTÍNEZ TOSAR, LEANDRO J. AND BOCCACCIO, GRACIELA L.
Lugar:
Pinamar - Argentina
Reunión:
Congreso; XLI Reunión Anual SAIB; 2005
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;
mso-font-charset:0;
mso-generic-font-family:roman;
mso-font-pitch:variable;
mso-font-signature:-1610611985 1107304683 0 0 159 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-unhide:no;
mso-style-qformat:yes;
mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
mso-bidi-font-size:10.0pt;
font-family:"Arial","sans-serif";
mso-fareast-font-family:"Times New Roman";
mso-bidi-font-family:"Times New Roman";
mso-ansi-language:ES;
mso-fareast-language:ES;}
p.MsoBodyTextIndent2, li.MsoBodyTextIndent2, div.MsoBodyTextIndent2
{mso-style-noshow:yes;
mso-style-unhide:no;
mso-style-link:"Sangría 2 de t\. independiente Car";
margin-top:0cm;
margin-right:0cm;
margin-bottom:0cm;
margin-left:14.2pt;
margin-bottom:.0001pt;
mso-pagination:none;
font-size:12.0pt;
mso-bidi-font-size:10.0pt;
font-family:"Arial","sans-serif";
mso-fareast-font-family:"Times New Roman";
mso-bidi-font-family:"Times New Roman";
mso-ansi-language:EN-US;
mso-fareast-language:ES;}
span.Sangra2detindependienteCar
{mso-style-name:"Sangría 2 de t\. independiente Car";
mso-style-noshow:yes;
mso-style-unhide:no;
mso-style-locked:yes;
mso-style-link:"Sangría 2 de t\. independiente";
mso-ansi-font-size:12.0pt;
font-family:"Arial","sans-serif";
mso-ascii-font-family:Arial;
mso-hansi-font-family:Arial;
mso-ansi-language:EN-US;
mso-fareast-language:ES;}
.MsoChpDefault
{mso-style-type:export-only;
mso-default-props:yes;
font-size:10.0pt;
mso-ansi-font-size:10.0pt;
mso-bidi-font-size:10.0pt;}
@page Section1
{size:612.0pt 792.0pt;
margin:70.85pt 3.0cm 70.85pt 3.0cm;
mso-header-margin:36.0pt;
mso-footer-margin:36.0pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
Eukaryotic cells respond to stress
by dramatically altering mRNA metabolism in phenomena such as localization,
translational state and rate of decay. The most studied event involved in these
changes is the assembly of stress granules (SG). We have shown previously that
under oxidative stress, mammalian Staufen 1 undergoes aggregation in SG (Thomas
et al. MBC 2005). The colocalization of Staufen 1 with SG markers was also
demonstrated in many different stress conditions and cell types, and thus the
possibility for an active role in the stress response was suggested. In this
work we show that cell lines overexpressing Staufen 1 fail to assemble SG, in
response to different stressors. However, phosphorilation of eIF2-a a required event in the stress response cascade, and in SG formation is normal in Staufen 1 overexpressing cells,
suggesting a downstream action of Staufen. We used several constructs bearing
deletions, isolated double-stranded RNA-binding domains (dsRBDs) and oligomeric
domains, to identify the protein region(s) responsible for SG inhibition. This
study presents the first physiological evidence of a role for Staufen 1 in the
regulation of SG assembly.
