ANTIFUNGAL ACTIVITY OF EXTRACTS FROM Amphilophium cynanchoides ON Aspergillus niger AND Aspergillus carbonarius

APUD, G. R.; AREDES FERNANDEZ, P.A.; SAMPIETRO, D. A.; VATTUONE, M.A.; CATALAN,C.A.N.

TUCUMAN

Congreso; XXXI ANNUAL SCIENTIFIC MEETING OF TUCUMAN BIOLOGY ASSOCIATION; 2014

TUCUMAN BIOLOGY ASSOCIATION

The Aspergillus black rot causes grape contamination with mycotoxins such as ochratoxin A. There is a need for new molecules to control this disease. In this work, the antifungal activity of leaves and stems of Amphilophium cynanchoides was evaluated on growth of A. niger y A. carbonarius. Aerial parts of Amphilophium cynanchoides collected in Trancas (Tucumán) were separated in stems and leaves, grounded and extracted with a sequence of solvents (dichloromethane, ethyl acetate and methanol). The organic extracts were evaporated to dryness, and a known amount of each dry residue was dissolved in ethanol, obtaining the fractions fChCl2, fAcet and fMeOH, respectively. The last ones were assayed by the micro-dilution method in YES semi-liquid medium (2500-2.4ppm) on 104 conidia/mL. Fungal biomass was determined by reading absorbance at 620nm. The IC50 values of fChCl2, fAcet and fMeOH from leaves and fMeOH from stem were higher than 2500ppm for both fungal species. The fChCl2 and fAcet from stems showed CI50s of 2178ppm and 1631ppm on A. niger and no activity on A. carbonarius. Our results indicated that the stem extracts of low and middle polarity containedallelochemicals with selective antifungal activity on A. niger.