INVESTIGADORES
ALVAREZ Cecilia Ines
congresos y reuniones científicas
Título:
Inflammation mediators regulate the LRP-1 Expression in J774 Cells
Autor/es:
CESCHIN D, CÁCERES L, SÁNCHEZ MC, ALVAREZ C, CHIABRANDO G.
Lugar:
Igauzú, Misiones. Argentina
Reunión:
Congreso; SAIB; 2004
Resumen:
In atherosclerosis, angiogenesis and tumoral metastasis occur a
focal degradation of extracellular matrix components by a broad
spectrum of proteinases. Anyway, anti-proteolytic molecules,
including proteinase inhibitors and endocytic receptors, play key
roles to control these processes. In this sense, LRP-1, a
multifunctional endocytic receptor that belongs to the LDL receptor
gene family, has been reported to modulate the extracellular
proteolytic activity in inflammation. Although it is known that
certain inflammatory mediators, including Insulin and Interferon-
ã (INF-ã) or bacterial lipopolysacharides (LPS), could affect the
biological function of LRP-1, the molecular mechanisms are not
clearly understood. In this work we study the regulatory effect of
inflammatory mediators and Insulin on LRP-1 expression in J774
macrophage-derived cell line. By western blotting and RT-PCR
we demonstrate that both insulin and inflammatory mediators -
obtained from a conditioned media of NIH.3T3 fibroblast-derived
cell line (MCN) down-regulate LRP-1 to protein and RNA level.
In addition, by immunofluorescence assay we observe that insulin
and MCN modify the endocytic activity of LRP-1 in J774 cells. In
conclusion in this work we demonstrate that inflammatory
mediators can affect the function of LRP-1 by regulating both the
expression and endocytosis activity of this receptor.