CONICET | Buscador de Institutos y Recursos Humanos

Expresión de Receptores de Neurotrofinas de alta afinidad (proteínas Trks) de Sus scrofa domesticus en Órganos Linfáticos Secundarios. Gauna Añasco, L; Dimatteo, AM.; Soñez, MC; Lombardo, DM., Vega, JA., von Lawzewitsch, I. There are evidences that neurotrophins can work on other tissues that express mRNA and /or their receptors, as rats, mice and man lymphatic system. This investigation goal was to determine Trk receptors localization in pigs secondary lymphatic organs (spleen, lymphnodules, tonsils and Peyers patches).Samples from young pigs (n=20) were fixed in 10% buffered formaldehyde and processed by routine histological techniques. Trks proteins were labeled with rabbit polyclonal antibodies diluted 1:100(Santa Cruz Biotechnology, CA, USA), employing the immunocytochemistry avidine biotine .peroxidase method (ABC). Spleen: Trk A and Trk B were localized in white pulp and in blood vessels inside lymphoid follicles. TrkC was identified in capsule, trabecules, and lymphoid tissue of the red pulp. Lymph nodules follicles: Trk C was found in cortical lymphoid tissue and capsule. Trk A and Trk B had negative immunoreactivity. Palatine tonsils: Trk were shown in lymph follicles center. Trk B showed negative immunoreactivity. We conclude that Trk A and Trk C studied expression in pigs secondary lymphatic organs would indicate the probably action of NGF and NT-3 growing factors , while Trk B was identified only in spleen this would suggest a possible role of BDNF and NT4/5 as growing factors in this organ, for maintenance of cellular populations.

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