INVESTIGADORES
ACOSTA Gabriela Beatriz
congresos y reuniones científicas
Título:
The activation of NF-KB is required for the long term memory consolidation in the passive avoidance learning mice.
Autor/es:
FREUDENTHAL R, BOCCIA MM, ACOSTA GB, BLAKE, MG, MERLO E, BARATTI CM, ROMANO A
Lugar:
Mar del Plata, 16-20 de Noviembre de 2004.
Reunión:
Congreso; 1er Congreso Conjunto de Sociedades Biomédicas.; 2004
Institución organizadora:
SAFE, SAN, SAB, SAI, SAIC, SAB, SAF
Resumen:
Although is generally accepted that memory consolidation
requires gene expression, only few transcription factors (TFs)
were clearly demonstrated to be specifically involved in this
process. Increasing research data point to the participation of Rel/
NF-kB family of TFs in memory and neural plasticity. Here we
found that icv post-training administration of sulfasalazine, an
inhibitor of NF-kB, impairs long term memory in the one-trial
stepthrough inhibitory avoidance paradigm in CF-1 mice.
Furthermore, we employed another independent strategy to inhibit
NF-kB in the mouse brain, administering a double stranded DNA
oligonucleotide containing the NF-kB consensus sequence (kBdecoy).
The icv injection of 60 ug of kB decoy 2 h before training
induced amnesia, while 60 ug of double stranded DNA
oligonucleotides with one base mutation in the consensus
sequence (mut-kB-decoy) did not affected long-term memory. NFkB
was inhibited in hippocampus 2 h after icv injection of 60 ug
of kB-decoy, but no inhibition was found 2 h after mut-kB-decoy
injection, estimated by electrophoretic mobility shift assay
(EMSA). A temporal course of hippocampal NF-kB activity after
training was determining by EMSA. Unexpectedly, an inhibition
of NF-kB was found 15 min after training session in the shocked trained
group but not in the unshoked-control group. Forty five
min after training, hippocampal NF-kB was activated in both
shocked and unshocked groups, returning to basal levels 2 hs
after training. Based on the latter result, we propose that the
activation of NF-kB in hippocampus is part of the molecular
mechanism involved in the storage of contextual features that
constitutes the conditioned stimulus representation. The results
presented here provide the first evidence supporting that NF-kB
activity is regulated in hippocampus during consolidation,
stressing the role of this TF as a conserved molecular mechanism
for memory storage.