INVESTIGADORES
IBAÑEZ Fernando Julio
congresos y reuniones científicas
Título:
IMPORTANCE OF GLUTATHIONE IN GROWTH OF PEANUT RHIZOBIA UNDER CADMIUM EXPOSURE
Autor/es:
BIANUCCI E, IBAÑEZ F, FABRA A, CASTRO S.
Lugar:
Mar del Plata
Reunión:
Congreso; XLIII SAIB; 2007
Institución organizadora:
SAIB
Resumen:
IMPORTANCE OF GLUTATHIONE IN GROWTH OF
PEANUTRHIZOBIAUNDERCADMIUMEXPOSURE
Bianucci E, IbañezF, Fabra A, Castro S.
Fac Cs Exactas, Fco-Qcas y Nat UNRC, 5800 Río Cuarto,
Córdoba, Argentina. E-mail: elianabianucci@yahoo.com.ar
Glutathione (GSH) is one of the best known non-enzymatic
defense systems.We demonstrated that GSH plays a crucial role in
the defense of Bradyrhizobium sp. SEMIA 6144 against different
environmental stresses. For that, a GSH-deficient mutant (B. sp
6144-S7Z) was obtained by disruption of A gene which
encodes the enzyme glutamylcysteine synthetase. Taking into
account that cadmium (Cd) is a toxic heavy metal that induces
oxidative stress in microorganisms, our objective was to
investigate the effect of this metal on the growth and survival of .
sp recommended as inoculants to peanut as well as the role of GSH
in the strategy of tolerance to Cd. The wild-type and GSH-deficient
mutant of B. sp. SEMIA6144 and B. sp. C-145 strains were grown
at different concentrations of Cd (0-15 μM) in the YEM medium.
B. sp. SEMIA6144 grew up to 15 μM Cd without difference in the
GSH content respect to control meanwhile the mutant strain was
unable to grow at 15 μM Cd. B. sp. C-145 showed a 75% of
decrease of growth with an increase in the GSH content at 15 μM
Cd. This result could suggest that the GSH content was not enough
to avoid the deleterious effect of Cd on the growth of B. sp. C-145.
In conclusion, our findings show that the strains used as field
peanut inoculants differ in the tolerance to Cd being B. sp. SEMIA
6144 tolerant and B. sp. C-145 sensitive.Glutathione (GSH) is one of the best known non-enzymatic
defense systems.We demonstrated that GSH plays a crucial role in
the defense of Bradyrhizobium sp. SEMIA 6144 against different
environmental stresses. For that, a GSH-deficient mutant (B. sp
6144-S7Z) was obtained by disruption of A gene which
encodes the enzyme glutamylcysteine synthetase. Taking into
account that cadmium (Cd) is a toxic heavy metal that induces
oxidative stress in microorganisms, our objective was to
investigate the effect of this metal on the growth and survival of .
sp recommended as inoculants to peanut as well as the role of GSH
in the strategy of tolerance to Cd. The wild-type and GSH-deficient
mutant of B. sp. SEMIA6144 and B. sp. C-145 strains were grown
at different concentrations of Cd (0-15 μM) in the YEM medium.
B. sp. SEMIA6144 grew up to 15 μM Cd without difference in the
GSH content respect to control meanwhile the mutant strain was
unable to grow at 15 μM Cd. B. sp. C-145 showed a 75% of
decrease of growth with an increase in the GSH content at 15 μM
Cd. This result could suggest that the GSH content was not enough
to avoid the deleterious effect of Cd on the growth of B. sp. C-145.
In conclusion, our findings show that the strains used as field
peanut inoculants differ in the tolerance to Cd being B. sp. SEMIA
6144 tolerant and B. sp. C-145 sensitive.