INVESTIGADORES
GONZALEZ FLECHA Francisco Luis
congresos y reuniones científicas
Título:
Red blood cell membrane dynamics explored by confocal microscopy
Autor/es:
MARTÍN DODES TRAIAN; VALERIA LEVI; F. LUIS GONZÁLEZ FLECHA
Lugar:
Tucuman
Reunión:
Congreso; XLI Reunion Anual de la Sociedad Argentina de Biofisica; 2012
Institución organizadora:
Sociedad Argentina de Biofisica
Resumen:
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Our knowledge of the
organization and function of biological membranes has changed from
the original model of Singer and Nicholson. It is now well accepted
that membrane components are not randomly organized but they assemble
forming domains of different compositions, sizes and dynamics which
are essential for the functionality of the membrane (1). It was
reported that the reaction of glucose with biological molecules
(non-enzymatic glycosylation) affects the physicochemical, and
occasionally also the biological properties of the target molecules
(2). Several studies have demonstrated that proteins and
amino-phospholipids react in vivo with glucose (3) generating Amadori
products and advanced glycation endproducts, which have been
implicated in the pathogenesis of diabetes and normal aging (4).
Using confocal microscopy and the fluorescent probes C-Laurdan (5)
and Rhodamine-DPPE, we explored membrane dynamics and microfluidity
in human red blood cells (RBC). The incubation media were carefully
designed for long term incubations of RBC exposed to either
physiological or high concentrations of glucose at 37 C and pH 7.4.
It is known that in these conditions glucose can react with the amino
groups of lysine residues in proteins and amino-phospholipids
generating Amadori products. The result is the decrease in the
activity of some membrane enzymes and the weakening of lipid protein
interactions (6). The aim of this project is to gain insight into the
effects of glycation on biological membranes in cells.