INCREASED OF HUMAN ERYTHROCYTES DEFORMABILITY BY DIACYLGLICEROL AND PHOSPHATIDIC ACID

MONESTEROLO NE; AMAIDEN MR; SANTANDER VS; ARCE CA; CASALE CH

Mendoza

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2012

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

During the hypertension numerous alterations of the rheological properties of theblood have been described, such as erythrocytes deformability. This parameter is determined partially bythe viscoelastic properties of cell membrane, which depend on his lipid composition and the intracellularcalcium concentration. Previously, we demonstrated that the content of tubulin in the membrane affects thedeformability of erythrocytes. On the other hand, we have described that plasma membrane calcium ATPase(PMCA), a P-ATPase, form a complex with tubulin, as a consequence enzymatic activity results inhibited;this regulation is dependent on the lipidic environment where PMCA is immersed. On the base of theseantecedents, in the present work we study if changes in lipidic environments are able to affect erythrocytesdeformability in normotensive and hypertensive subjects. Results shown that the addition of 50 uM ofdiacylglicerol or phosphatidic acid increases erythrocyte deformability in normotensive and hypertensivesubjects. Moreover, after lipid treatment are not observed significantly differences in the deformability oferythrocytes of normotensive and hypertensive subjects. Pretreatment of erythrocytes with activators einhibitors of PMCA and Na+,K+-ATPase shown that effects in erythrocyte deformability induced by lipidswould be mediated by modifications in such enzymatic activities.